retired
General Surgery
Dr. Michael N. Margolies, MD
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Dr. Michael N. Margolies works in the field of General Surgery. He attended Columbia University College of Physicians & Surgeons. Columbia University College of Physicians & Surgeons is a one of the best medicals schools. Columbia University College of Physicians & Surgeons is ranked 8 in research and 52 in primary care medicine He received 1 awards: "Fellow (FACS)". Michael Margolies is also a published physician. He has 100 paper published. The most recent paper is: Structural requirements for a specificity switch and for maintenance of affinity using mutational analysis of a phage-displayed anti-arsonate antibody of Fab heavy cha...
Publications
- Modifying specificity of antidigoxin antibodies using insertional mutagenesis.
- Ouabain-binding protein(s) from human plasma.
- Complementary combining site contact residue mutations of the anti-digoxin Fab 26-10 permit high affinity wild-type binding.
- Normative study of accommodative facility in elementary schoolchildren.
- Solitary rheumatoid lesion in the lung.
- A normative study of step vergence in elementary schoolchildren.
- Selection of high affinity p-azophenyarsonate Fabs from heavy-chain CDR2 insertion libraries.
- Structural analysis of mutants of high-affinity and low-affinity p-azophenylarsonate-specific antibodies generated by alanine scanning of heavy chain complementarity-d...
- A single H:CDR3 residue in the anti-digoxin antibody 26-10 modulates specificity for C16-substituted digoxin analogs.
- Phage display-selected sequences of the heavy-chain CDR3 loop of the anti-digoxin antibody 26-10 define a high affinity binding site for position 16-substituted analog...
- Selection of peptidic mimics of digoxin from phage-displayed peptide libraries by anti-digoxin antibodies.
- Effect of pressure on antigen-antibody complexes: modulation by temperature and ionic strength.
- Aromatic residues mediate the pressure-induced association of digoxigenin and antibody 26-10.
- An approach for preventing recombination-deletion of the 40-50 anti-digoxin antibody V(H) gene from the phage display vector pComb3.
- Monoclonal antibodies that distinguish between two related digitalis glycosides, ouabain and digoxin.
- Structural requirements for a specificity switch and for maintenance of affinity using mutational analysis of a phage-displayed anti-arsonate antibody of Fab heavy cha...
- Identification of a model cardiac glycoside receptor: comparisons with Na+,K+-ATPase.
- High affinity anti-digoxin antibodies as model receptors for cardiac glycosides. Comparisons with Na+,K(+)-ATPase.
- Differences in sequence-specific expression of two anti-arsonate Fabs in E. coli.
- Antibody binding sites.
- Contribution of heavy chain junctional amino acid diversity to antibody affinity among p-azophenylarsonate-specific antibodies.
- Modulation of antibody affinity by a non-contact residue.
- Conservation of binding site geometry among p-azophenylarsonate-specific antibodies.
- Defining the structural correlates responsible for loss of arsonate affinity in an IDCR antibody isolated from an autoimmune mouse.
- A single engineered amino acid substitution changes antibody fine specificity.
- 26-10 Fab-digoxin complex: affinity and specificity due to surface complementarity.
- Effect of heavy chain signal peptide mutations and NH2-terminal chain length on binding of anti-digoxin antibodies.
- Modulation of antibody affinity by an engineered amino acid substitution.
- Structure and specificity of the anti-digoxin antibody 40-50.
- Heavy chain position 50 is a determinant of affinity and specificity for the anti-digoxin antibody 26-10.
- Contribution of antibody heavy chain CDR1 to digoxin binding analyzed by random mutagenesis of phage-displayed Fab 26-10.
- A misplaced caval filter: its removal from the heart without cardiopulmonary bypass.
- Two structurally distinct and independently regulated idiotypic families associated with the A/J response to azophenylarsonate.
- Hepatic portal venous gas in diverticulitis: survival in a steroid-treated patient.
- Analysis of immune response at the molecular level.
- Crystallization of the Fab fragment of a monoclonal anti-digoxin antibody and its complex with digoxin.
- Structural diversity among anti-p-azophenylarsonate monoclonal antibodies from A/J mice; comparison of Id- and Id+ sequences.
- Quantitative solid-phase Edman degradation for evaluation of extended solid-phase peptide synthesis.
- Hybridoma proteins expressing the predominant idiotype of the antiazophenylarsonate response of A/J mice.
- Analysis of the anti-azobenzenearsonate response at the molecular level.
- Reversal of lethal digoxin toxicity in guinea pigs using monoclonal antibodies and Fab fragments.
- Complete amino acid sequence of the heavy-chain variable region from an A/J mouse antigen-nonbinding monoclonal antibody bearing the predominant arsonate idiotype.
- Use of o-phthalaldehyde to reduce background during automated Edman degradation.
- Structural correlates of idiotypy in the arsonate system.
- Amino acid sequence of the heavy chain variable region from the A/J mouse anti-arsonate monoclonal antibody 36-60 bearing a minor idiotype.
- Amino acid sequence of the light chain variable region from a mouse anti-digoxin hybridoma antibody.
- Expression and rearrangement of homologous immunoglobulin VH genes in two mouse strains.
- Complete heavy and light chain variable region sequence of anti-arsonate monoclonal antibodies from BALB/c and A/J mice sharing the 36-60 idiotype are highly homologous.
- Monoclonal antibodies as physiologic probes.
- Fine specificity of idiotope suppression in the A/J anti-azophenylarsonate response.
- Immunoglobulin idiotype and anti-anti-idiotype utilize the same variable region genes irrespective of antigen specificity.
- High-affinity monoclonal antibodies to the cardiac glycoside, digoxin.
- Isolation of an active heavy-chain variable domain from a homogeneous rabbit antibody by cathepsin B digestion of the aminoethylated heavy chain.
- Isolation of the fourth (isomerase) of histidine biosynthesis from Salmonella typhimurium.
- Expression of a hybrid immunoglobulin-T cell receptor protein in transgenic mice.
- The amino acid residues at the VH-D-JH junctions affect the affinity of anti-p-azophenylarsonate antibodies.
- Structural basis for the species selectivity of a fibrin-specific monoclonal antibody.
- Parallel evolution of antibody variable regions by somatic processes: consecutive shared somatic alterations in VH genes expressed by independently generated hybridoma...
- Crystal structure of the antigen-binding fragment of the murine anti-arsonate monoclonal antibody 36-71 at 2.9-A resolution.
- Phosphorylation state of pro-atrial natriuretic factor in rat atrial secretory granules.
- Heavy and light chain contributions to antigen binding in an anti-digoxin chain recombinant antibody produced by transfection of cloned anti-digoxin antibody genes.
- A V kappa-J kappa junctional change in an antidigoxin recombinant antibody destroys digoxin-binding activity.
- Clustered H chain somatic mutations shared by anti-p-azophenylarsonate antibodies confer enhanced affinity and ablate the cross-reactive idiotype.
- Structural characterization of H chain-associated idiotopes of anti-p-azophenylarsonate monoclonal antibodies.
- Three-dimensional structure of murine anti-p-azophenylarsonate Fab 36-71. 2. Structural basis of hapten binding and idiotypy.
- Three-dimensional structure of murine anti-p-azophenylarsonate Fab 36-71. 1. X-ray crystallography, site-directed mutagenesis, and modeling of the complex with hapten.
- Altered hapten recognition by two anti-digoxin hybridoma variants due to variable region point mutations.
- A spontaneous variant of an antidigoxin hybridoma antibody with increased affinity arises from a heavy chain signal peptide mutation.
- The application of 0.1 M quadrol to the microsequence of proteins and the sequence of tryptic peptides.
- Diversity of light chain variable region sequences among rabbit antibodies elicited by the same antigens.
- Diversity among rabbit antibody light chain amino terminal sequences.
- Malignant seeding of the tract after thin-needle aspiration biopsy.
- Computerized tomography of in vitro Abdominal organs--effect of preservation methods on attenuation coefficient.
- Increased efficiency in solid-phase Edman degradation of synthetic peptidyl-resins using an oxymethylphenylacetamidomethyl-linkage.
- Amino acid sequence of normal (microheterogeneous) porcine immunoglobulin lambda chains.
- The structural basis of rabbit VH allotypes: serologic studies on a1 H chains with defined amino acid sequence.
- The interdomain disulfide bond of a homogeneous rabbit pneumococcal antibody light chain.
- Peptides: an analytical method for their resolution by polyacrylamide gel electrophoresis applicable to a wide range of sizes and solubilities.
- Hemorrhage with pelvic fractures: efficacy of transcatheter embolization.
- Isolation and characterization of an active variable domain from a homogeneous rabbit antibody light chain.
- Amino acid sequence of the heavy-chain variable region of the crystallizable human myeloma protein Dob.
- Light chain variable region sequence of rabbit antipneumococcal type III polysaccharide antibody 3368.
- Preparation of an active Fd fragment by cyanogen bromide cleavage of an IgG heavy chain from a homogeneous rabbit antibody.
- Antibodies of restricted heterogeneity directed against the cardiac glycoside digoxin.
- Origins of antibody diversity: insight gained from amino acid sequence studies of elicited antibodies.
- Purification, specificity, and hypervariable region sequence of anti-pneumococcal polysaccharide antibodies elicited in a single rabbit.
- Arteriography in the management of hemorrhage from pelvic fractures.
- Correlation between mutation type and the production of cross-reacting material in mutants of the A gene of the histidine operon in Salmonella typhimurium.
- Physical and chemical characterization of the isomerase of histidine biosynthesis in Salmonella typhimurium.
- Relative translation frequencies of the cistrons of the histidine operon.
- Renal and gastrointestinal excretion of ouabain in dog and man.
- Arteriographic management of hemorrhage following pelvic fracture.
- Arteriography in pelvic fractures with massive hemorrhage.
- Isolation and characterization of structurally homogeneous antibodies from antipneumococcal sera.
- Amino acid sequence of rabbit pneumococcal antibody. I. Light-chain cysteine-containing peptides.
- Variation in the primary structure of antibodies during the course of immunization.
- Binding and structural diversity among high-affinity monoclonal anti-digoxin antibodies.
- The association of various D elements with a single-immunoglobulin VH gene segment: influence on the expression of a major cross-reactive idiotype.
- Isolation, characterization, and distribution of an unusual pancreatic human secretory protein.
Schools
Columbia University College of Physicians And Surgeons
Massachusetts General Hospital
National Institutes Of Health
Procedures Preformed
- Dressing and-or Debridement of Wound, Infection, or Burn (incl. Negative Pressure Wound Therapy)
Conditions Treated
- Abdominal Pain
- Anorectal Abscess
- Breast Cancer
- Hemorrhoids
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Doctors Specialties
- General Surgery
Accepted Insurances
Awards
- Fellow (FACS)
Education
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Massachusetts General Hospital -
Columbia University College of Physicians & Surgeons
